JoVE Bioengineering

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

1, 1, 1, 1, 1, 1

1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Juelich GmbH

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    Summary

    In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.

    Date Published: 12/06/2013, Issue 82; doi: 10.3791/50560

    Cite this Article

    Gruenberger, A., Probst, C., Heyer, A., Wiechert, W., Frunzke, J., Kohlheyer, D. Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation. J. Vis. Exp. (82), e50560, doi:10.3791/50560 (2013).

    Abstract

    In this protocol the fabrication, experimental setup and basic operation of the recently introduced microfluidic picoliter bioreactor (PLBR) is described in detail. The PLBR can be utilized for the analysis of single bacteria and microcolonies to investigate biotechnological and microbiological related questions concerning, e.g. cell growth, morphology, stress response, and metabolite or protein production on single-cell level. The device features continuous media flow enabling constant environmental conditions for perturbation studies, but in addition allows fast medium changes as well as oscillating conditions to mimic any desired environmental situation. To fabricate the single use devices, a silicon wafer containing sub micrometer sized SU-8 structures served as the replication mold for rapid polydimethylsiloxane casting. Chips were cut, assembled, connected, and set up onto a high resolution and fully automated microscope suited for time-lapse imaging, a powerful tool for spatio-temporal cell analysis. Here, the biotechnological platform organism Corynebacterium glutamicum was seeded into the PLBR and cell growth and intracellular fluorescence were followed over several hours unraveling time dependent population heterogeneity on single-cell level, not possible with conventional analysis methods such as flow cytometry. Besides insights into device fabrication, furthermore, the preparation of the preculture, loading, trapping of bacteria, and the PLBR cultivation of single cells and colonies is demonstrated. These devices will add a new dimension in microbiological research to analyze time dependent phenomena of single bacteria under tight environmental control. Due to the simple and relatively short fabrication process the technology can be easily adapted at any microfluidics lab and simply tailored towards specific needs.

    Introduction

    Time-lapse microscopy is a powerful tool for studying living cells in vivo1. Meanwhile commercially available fully automated microscopy platforms including thermally induced focus drift compensation are commonly applied in biological research to study time-dependent phenomena, ranging from cancer and neuron cell research over tissue engineering and dynamic studies with single yeast or bacterial cells2-6.

    Typically transparent well plates, agar-pads, or simply microscopy slides are applied to provide cell culture environments during time-lapse imaging7. Even though suitable for certain research, these simple systems have very limited control over environmental conditions and do not allow for more complex perturbations or well defined and fast medium changes. Disposable microfluidic chip devices produced by mass production have been introduced to the market recently but are mostly tailored towards larger eukaryotic cell types4. Although growth can be followed, well defined growth investigations concerning, e.g. precise cell trapping, colony size, growth direction, and the ability for cell removal are limited. Microfluidic habitats and reactors, in which bacteria cells are cultured in 3D environments have been developed8-10, have drawbacks when dealing with quantitative studies at the single-cell level. While overall population heterogeneity can be analyzed, many cell parameters cannot be determined accurately with single-cell resolution since growth is not restricted to monolayers.

    This limitation triggered the development of microsystems enabling the cultivation of cells in well-defined channels and habitats with cells growing in flat monolayers with single cell resolution and especially tight control over media supply and environmental conditions6,11,12. Few examples of microfluidic systems for the cultivation of bacterial cells have been demonstrated12-14. Bacteria typically exhibit very fast growth rates and require microfluidic structures in the range of few micrometers and below, especially when cell monolayers are desired for microscopy. Keymer et al. demonstrated growth and spreading of E. coli strains in microfabricated landscapes15,16. Since they were interested in population dynamics they did not investigate with single-cell resolution.

    We have developed the picoliter bioreactor (PLBR)13, which is currently applied to investigate various biotechnological performance indicators such as growth17 and fluorescence coupled productivity analysis on single-cell level18,19. The present microfluidic device allows environmental reactor control at a defined culture volume of approximately one picoliter and continuous single-cell observation simultaneously. In comparison to open monolayer box systems11,14, where one or two sides are open to the media supply channel, the PLBR allows for controlled trapping and culturing. The design permits long term cultivation of bacteria without the risk of several adjacent colonies forming one large population. Furthermore, the system incorporates cultivation regions of 1 µm height (in the order of the cell diameter) to restrict bacteria growth to cell monolayers. In contrast, the supply channels are 10 fold deeper to minimize hydraulic resistance.

    In comparison to miniaturized batch cultivation systems20 the present system allows the cultivation with constant environmental parameters due to continuous media flow. Furthermore, environmental parameters such as medium composition, temperature, flow rates and gas exchange can be easily controlled and changed within seconds. This allows for specific investigations of cellular response to environmental changes concerning for example nutrient availability or stress stimuli. The demand for reduced media volumes, namely in the range of few microliters only, enable researchers to perform novel studies, e.g. the perturbation of cells during time-lapse imaging with supernatant of large-scale experiments unraveling cell response under these specific environmental conditions17. The picoliter bioreactor provides researchers with a robust system that tightly controls biophysical conditions and is operated using high precision syringe pumps and automated bright field and fluorescence microscopy for time-lapse imaging. Here, we report a complete protocol including device design, fabrication, and exemplary applications.

    Protocol

    1. Wafer Fabrication

    1. Design the microfluidic device containing inlets, outlets, main channels and the PLBRs (Figure 1A) using CAD software.
    2. The design presented in this protocol (Figure 2) consists of two seeding inlets, a gradient generator for mixing of two different substrates, one outlet, and six arrays of PLBRs. Each array contains 5 PLBRs, resulting in 30 parallel PLBRs inside one microfluidic device.
    3. Create a lithography photomask containing the desired chip layouts (Figure 1B). The photomask was produced in-house by electron beam writing with submicron resolution. The mask used was composed of a chromium layer on a 5 in2 glass plate.
      Note: perform all following steps under cleanroom class 100 conditions or better (a process flowchart is shown in Figures 3A and 3B).
    4. Clean a 4 in silicon wafer with piranha (10:1 ratio of sulfuric acid and hydrogen peroxide) and hydrofluoric acid for several minutes (Caution: hazardous chemicals). Rinse with deionized (DI) water for approximately 10 sec.
    5. Dehydrate wafer for 20 min at 200 °C.
    6. Spin coat 1 µm SU-8 2000.5 photoresist onto the wafer (1st layer) (4 ml resist, spin 10 sec with, v = 500 rpm, and a = 100 rpm/sec, spin 30 sec with v = 1,000 rpm and a = 300 rpm/sec).
    7. Place the coated wafer on a hotplate at 95 °C to drive off excess solvent (1.5 min at 65 °C, 1.5 min at 95 °C, and 1 min at 65 °C; ideally use two hotplates).
    8. Insert 1st layer photomask (here the trapping regions of the picoliter reactors) and wafer inside the mask aligner and expose wafer to 350-400 nm (vacuum contact, 64 mJ/cm², t = 3 sec, I = 7 mW/cm²).
    9. Perform post exposure bake on a hotplate at 95 °C to initiate the polymerization of SU-8 (1 min at 65 °C, 1 min at 95 °C, and 1 min at 65 °C). Note: after this step the structures in the SU-8 layer can be seen.
    10. Place the wafer in a SU-8 developer bath for 1 min and transfer the wafer into a second container with fresh SU-8 developer for few seconds.
    11. Rinse the wafer in isopropanol to remove SU-8 developer and dry wafer using nitrogen flow of wafer spinner.
    12. Hard bake the wafer for 10 min at 150 °C.
    13. Spin coat 9 µm SU-8 2010 photoresist onto the wafer (2ndlayer) (dispense 4 ml resist, spin 10 sec with v = 500 rpm, a = 100 rpm/sec, and spin 30 s with v = 4,000 rpm, a = 300 rpm/sec).
    14. Place the wafer with SU-8 on a hotplate at 95 °C to drive off excess solvent (15 min at 65 °C, 45-60 min at 95 °C, and 10 min at 65 °C). Note: attention has to be paid to wrinkles and bubbles. If the wafer is heated to fast to 95 °C, evaporated solvent may be encapsulated in tiny gas bubbles.
    15. Insert photomask with the desired layout (here main channels for nutrient supply) and the wafer into the mask aligner and expose to 350-400 nm (hard contact, 64 mJ/cm², t = 10 sec, I = 7 mW/cm²)
    16. Perform post exposure bake on a hotplate at 95 °C to finalize the polymerization of SU-8 (5 min at 65 °C, 3:30 min at 95 °C, 3 min at 65 °C). Note: after this step the structures in the SU-8 layer can be seen.
    17. Place the wafer in a SU-8 developer bath for 45 sec, transfer the wafer into a second container with fresh SU-8 developer and develop for 60 sec.
    18. Rinse the wafer 20 sec with isopropanol to remove any SU-8 developer residue and dry wafer using pressured nitrogen.
    19. Finally hard bake the wafer at 150 °C. As a result the final wafer (Figure 1C) is obtained, which will be used as master mold for PDMS molding.
    20. Perform profilometer measurements (Figure 3C) to validate SU-8 structure heights. Note: inaccuracies in structure height may results in inefficient cell trapping or loss of cells during cultivation.

    2. Polydimethylsiloxane Chip Fabrication

    Note: All following steps should be ideally performed under laminar-flow conditions to prevent dust particles interfering with the fabrication procedure (A process flowchart is shown in Figure 4).

    1. Prepare a mixture of polydimethylsiloxane (PDMS) base and curing agent in a 10:1 ratio. Mix carefully until a homogenous solution is achieved which looks opaque. Prepare as much as required for the desired layer height (here 3 mm).
    2. Degas the PDMS mixture for approximately 30 min under slight vacuum until all bubbles have disappeared.
    3. Prepare molding device (or Petri dish) with appropriate SU-8 wafer and pour the PDMS mixture into it (Figure 1D).
    4. Bake the PDMS for 3 hr at 80 °C in the oven.
    5. Carefully peel off the PDMS slab from the wafer. Cut the slab into single chips using a clean and sharp scalpel.
    6. Wash the chips in a n-pentane bath for 90 min, followed by two acetone washing baths (90 min each). Dry the chips overnight to remove any solvent residue. Caution: perform the PDMS washing under a fume hood Note: during the n-pentane wash, monomers and dimers are removed from the cured PDMS and the chip size may temporarily double during washing procedure.
    7. Store the microfluidic PDMS chips in close containers until the final experiment.
    8. Just before the experiment, punch the inlet and outlet holes into the PDMS chip using a needle (or hole-puncher) with a slightly smaller diameter than the connectors that are used to connect tubing with PDMS chip.
    9. Clean the microfluidic PDMS chip carefully with isopropanol and use scotch tape to remove any dust particles which might stick on the structured PDMS side. Use the scotch tape several times until no particle can be seen on the chip.
    10. Clean a 170 µm thin glass slide with acetone and isopropanol successively. Finally clean with deionized water and dry with pressurized nitrogen.
    11. Before plasma-activation, warm up the plasma cleaner and run the plasma for approximately 300 sec. Plasma-oxidize glass slide and PDMS chip (Power 50 W, Time = 25 sec, oxygen flow rate = 20 sccm).
    12. Align the PDMS and glass chip before bonding. Finally, place the PDMS chip carefully onto the glass slide (Figure 1E). PDMS and glass will bond within seconds. Note: do not push with tweezers onto the top of the PDMS chip during the bonding process. This may lead to so called roof-collapsing of the channels and small structures.
    13. In order to strengthen the bond, bake the final PDMS-glass chip for 10 sec at 80 °C.

    3. Preparation of the Bacterial Culture

    Note: All cultivations should be prepared in sterile filtered medium to prevent accumulation of undesired particles, which may interfere during cultivation.

    1. Use an agar plate containing the desired organisms (here, C. glutamicum ATCC 13032) and inoculate one colony into 20 ml of fresh BHI medium, incubate overnight (≈ 8-14 hr) at 30 °C on a rotary shaker (120 rpm).
    2. Transfer 10 µl of the preculture into the desired medium (here CGXII21) which will be used during microfluidic cultivation and let the cell grow overnight at 30 °C on a rotary shaker (120 rpm).
    3. Transfer the desired amount of cell suspension (between 10-500 µl, depending on the start of the experiment) into the desired medium (here CGXII21) which will be used during microfluidic cell cultivation. Note: the best is to use cells from the early logarithmic phase for seeding. For C. glutamicum culture the best optical density (OD600) for seeding was between 0.5-2.
    4. Transfer 1 ml of the bacterial culture into a sterile 2 ml tube. Note: this should be done right after the microfluidic PDMS chip was assembled to minimize transfer time between shake flask and microfluidic cultivation. Typically the transfer time is around 15 min and should be kept as small as possible to prevent impact on metabolism caused by oxygen limitation and temperature changes.

    4. Experimental Setup

    Note: All steps are performed with an inverted microscope.

    1. Start microscope incubator 2 hrs before the experiment to warm-up the system. Note: the microscopy should be equipped with a full-size incubator to control temperature and if desired gas flow. Additional humidity control is not necessary since the chip system is continuously infused with media.
    2. Open incubator system, select the desired objective and if required add immersion oil onto the objective.
    3. Mount the chip inside the chip holder. If required fix the glass plate with adhesive tape in order to avoid chip any movement during stage operation.
    4. Center the sample on the microscope and focus onto the PLBR arrays.
    5. Connect inlets and outlets with appropriate tubing (Figure 1F). Connect tubing to a waste reservoir. A representative chip can be seen in Figure 4D.
    6. Insert syringes into pumps and start media flow. Use medium, buffer or if necessary coating solution and rinse the microfluidic channels for approximately 1 hr. Note: coating solution is used to coat channel walls to prevent unspecific cell adhesion.
    7. For E. coli, 0.1% solution of BSA is used to coat the channel walls. For C. glutamicum no coating is necessary. After the coating procedure flush the chip with medium prior cell seeding.
    8. Before cell seeding and cultivation, check that no leakage occurs and that the temperature is constant.

    5. Seeding of Bacterial Cells into the Microfluidic Device

    1. Make sure the desired bacteria solutions are available in appropriate syringes connected to tubing.
    2. Disconnect buffer or coating solution and connect the cell suspension to the chip. To minimize death volume, undesired air bubbles and to reduce experimental time, change the complete needle as well as tubing, rather than only the syringes.
    3. Infuse the cell suspension into the channels at a volumetric flow rate of 200 nl/min until most of the PLBRs are filled with the desired amount of cells (Figure 5A). Note: optimal seeding results depend on the bacterial strain, OD600, and growth medium of the preculture. These parameters have to be adapted to increase trapping efficiency and time until a sufficient number of cells are trapped in the reactor structures. For C. glutamicum, typically a cell suspension of OD600 0.5-2 was used; for E. coli the OD600 was between 0.5-1.
    4. If only a small number of PLBRs are filled, increase the flow rate to 800-1,200 nl/min.
    5. Disconnect the cell suspension and connect the growth medium to the chip (Figure 5B). Make sure that no air bubble is introduced during the medium change. Perfuse with fresh growth media at 100 nl/min.

    6. Time-lapse Imaging

    1. Select specific PLBRs for time-lapse imaging. Typically PLBRs are chosen that contain a single mother cell at the beginning of an experiment. The number of regions of interest that can be investigated in one experiment depends on the desired frame rate and microscopic setup.
    2. Select an appropriate frame rate depending of number of PLBRs. Make sure that the microscope can handle the desired amount of ROIs in the time lapse interval.
    3. Choose appropriate filter sets (here YFP). Automatically close the shutter during stage movement and after each time-lapse measurement to prevent chromophore bleaching.
    4. Configure the time-lapse microscopy sequence and start the experiment.
    5. After all PLBRs are overgrown, the experiment can be stopped, the microfluidic PDMS chip can be discarded and the experiment can be evaluated.

    7. Analysis

    Note: The following steps or parts of the procedure can be performed manually or by image analysis programs such as ImageJ, etc.

    1. Determine PLBRs of interest where the cultivation fulfills all desired criteria, e.g. number of mother cells, position of the mother cells, etc.
    2. To determine the growth rate of one microcolony count the number of cells in each time frame.
    3. Calculate the maximum growth rate by plotting time vs. ln (cell number). The slope of the plot represents the growth rate in [1/hr] (see Figure 6).
    4. Fluorescence data analysis strongly depends on the performed experiment. In this report, an example was chosen to illustrate colony-to-colony and cell-to-cell heterogeneity between different isogenic microcolonies (see Figure 7).

    Representative Results

    Device Fabrication

    The microfluidic PLBR system is fabricated by one layer of PDMS bonded onto a thin glass chip suited for high resolution microscopy. The fabrication consists of two main steps: firstly the fabrication of the replication master (Figures 1A, 1B, and 1C) and secondly the chip fabrication (Figures 1D, 1E, and 1F). According to the protocol, standard photolithographic microfabrication techniques are used to create the master mold. Laboratories without clean room facility can acquire commercially available customized SU-8 master molds. Using repetitive PDMS molding (Figures 1A, 1B, and 1C) hundreds of disposable chips can be produced. PDMS molding and chip assembly can be done in any lab and do not require clean room facilities, however, laminar airflow workplaces are favorable.

    The process starts with the design of the microfluidic chip system. Typically CAD software is used to design the microfluidic chip (Figure 1A). After CAD, a mask is generated by an e—beam writer (Figure 1B) with submicron resolution. In the present study a 5 in chromium mask was created which was used for the SU-8 wafer lithography. The final silicon—SU—8 wafer is used for PDMS molding (Figure 1D). After a baking step the PDMS slab is cut into chips which are irreversibly bonded onto the glass slides (Figure 1E). Finally the tubing is connected (Figure 1F).

    Figure 2 shows the design of the microfluidic system in detail. It consists of two seeding inlets, a gradient generator for mixing of different substrates or media and one outlet. The main channels have a dimension of 50 µm x 10 µm (W x H). Each device consists of six arrays of PLBRs, containing 5 PLBRs each. This results in 30 parallelized reactors inside one microfluidic device.

    Figure 3 illustrates the replication master production. As described in detail in the protocol, a first SU-8 layer is fabricated by SU-8 lithography (Figure 3A). A similar procedure is applied for the second layer (Figure 3B). To check the channel geometry we investigated the height of the PLBRs and main channels using a profilometer. In the example shown in Figure 3C, the first layer (the cultivation layer) was measured. Here the layer shows a consistent height of 1,200 nm, suitable for the cultivation of C. glutamicum in BHI medium.

    Figure 4 illustrates the PDMS molding procedure starting with PDMS mixing (Figure 4A) followed by the molding process (Figure 4B) and finally the bonding step (Figure 4C). Figure 4D shows the final microfluidic chip incorporating the 170 µm thick glass plate, PDMS chip (3 mm in height) with inlets and outlets and steel needles connected to tubing. After the experiment the chip can be disposed and no extensive cleaning is necessary. Furthermore, it is easy to assemble and handle. No complex and difficult filling procedure is necessary.

    Device Principle

    Figure 5 shows the working principle of the reactor system. Cells are infused into the microfluidic device and individual cells remain trapped inside the PLBR simply by cell-wall interactions. Due to the difference in hydrodynamic resistance of channel and PLBR, only minimal flow occurs inside the PLBR. After seeding of the PLBR (Figure 5A), the growth and observation phase is initiated with a change from bacteria solution to growth medium (Figure 5B). After the PLBRs are overgrown (Figure 5C) the experiment is typically stopped and time-lapse images can be analyzed. For the trapping mechanisms and flow profile within the PLBR the reader is referred to Grünberger et al.13 for more details.

    Growth Rate Analysis

    The present system can be applied to study various bacterial species with respect to different biological parameters such as growth, morphology, or a fluorescent signal. In a first example C. glutamicum, an industrially relevant production organism was cultured under standard cultivation conditions (T=30 °C, CGXII medium21). Figure 6A shows the growth curves derived from three isogenic microcolonies. Exponential growth is maintained until the PLBRs are filled indicating that no nutrient limitation occurs. Figure 6B displays four DIC time-lapse microscopy images of a growing C. glutamicum colony.

    Fluorescence Analysis

    For single-cell fluorescence microscopy, researchers often make use of specific fluorescent proteins, for example GFP or derivatives, to couple a specific phenotype of interest to a measurable output (a fluorescent signal). To demonstrate the applicability of the PBLR for fluorescence based time-lapse studies, we investigated the fluorescence emission of a C. glutamicum strain producing a plasmid-encoded YFP-TetR fusion protein under control of the Ptac promoter (pEKEx2-yfp-tetR)18,22. In the presence of low inducer (IPTG) concentrations, expression from Ptac is known to lead to significant cell-to-cell variation in isogenic bacterial populations. Starting from one preculture, the growth and single cell fluorescence was followed for several isogenic microcolonies. As it can be seen in Figure 7, we observed phenotypic heterogeneity between different microcolonies and heterogeneity at the single-cell level within colonies starting from one mother cell. One colony (Figure 7B, PLBR 1) showed almost no fluorescence emission, whereas cells of PLBR 2 exhibited a low fluorescence emission due to basal yfp-tetR expression from the Ptac promoter. In PLBR 3 fluorescence emission was considerable strong compared to the other colonies and a broad distribution of the population was observed. This example demonstrates the applicability of the PBLR for time-lapse fluorescence microscopy studies. In comparison to flow cytometry, in which the fluorescence of single cells can be determined at one time point, the present systems allows the tracking of cells and the study of single-cell fluorescence in real time over many generations.

    Figure 1
    Figure 1. Overview of PLBR chip production process. Master mold fabrication: starting with (A) Design, (B) Lithography mask fabrication, and (C) Wafer production. PDMS-glass chip production: starting with (D) of PDMS molding followed by (E) glass and PDMS bonding and (F) final chip assembly.

    Figure 2
    Figure 2. Design of the PLBR chip. (A) CAD drawing of the whole microfluidic chip; (B) Magnification of selected layout positions: The layout contains two medium inlets (a1), a gradient generator with mixing channels (a2) and 6 parallel PLBR arrays (b1). b2 shows one PLBR, which is embedded in a fluid channel with a width of 100 µm. The PLBR has an inner diameter of 40 µm and nutrient channels with 2 µm in width. The seeding inlet has a length of 40 µm. Pink color represents the first layer (trapping and cultivation region) and blue color represents the second layer (fluid transport).

    Figure 3
    Figure 3. Illustration of two layer wafer fabrication process. (A) Fabrication of the first layer containing trapping structures; (B) Fabrication of the second layer containing fluid channels, inlets and outlets; (C) Representative surface profiles of the first layer. In this case the height of the first layer was 1,200 nm and is used for the cultivation of C. glutamicum in complex medium.

    Figure 4
    Figure 4. Device fabrication and representative chip. Illustration of the PDMS molding process: (A) PDMS mixing and degassing; (B) PDMS molding; (C) mold release, cutting and chip bonding. Final chip (Reproduced with permission of the Royal Society of Chemistry13): (D) photograph of the PDMS chip with 2 inlets and 1 outlet; (E) CAD image of six parallel arrays containing 5 PLBRs each; (F) SEM image of one PLBR.

    Figure 5
    Figure 5. Working principle of the PLBR system. (A) Seeding phase; (B) Growth phase of the bacterial microcolonies; (C) Overflow phase. Reproduced with permission of the Royal Society of Chemistry13. http://dx.doi.org/10.1039/C2LC40156H.

    Figure 6
    Figure 6. Growth rate determination of C. glutamicum WT microcolonies. (A) Growth plot of three PLBR cultivations and resulting exponential curves (Parts reproduced with permission of the Royal Society of Chemistry)13. http://dx.doi.org/10.1039/C2LC40156H. (B) Time-lapse images of a growing C. glutamicum colony.

    Figure 7
    Figure 7. PBLR-based analysis of population heterogeneity. Shown is C. glutamicum expressing an yfp-tetR fusion under the control of the Ptac promoter (pEKEx2-yfp-tetR) in the absence of the inducer IPTG. (A) Experimental workflow; (B) Three isogenic microcolonies showing colony-to-colony heterogeneity and cell-to-cell heterogeneity; (C) Distribution of single-cell fluorescence within the respective microcolonies.

    Figure 8
    Figure 8. Scanning electron images of different PLBRs. SEM images showing seeding inlets for the optimization of trapping efficiency. (A) Lager seeding inlets (B) Smaller seeding inlets (C) Larger “open" seeding inlets (D) Two seeding inlets.

    Step Problem Possible Reason Solution
    Wafer Fabrication Trapped air bubbles in SU-8 during soft bake Increase of temperature to fast Bake at 95 °C and 65 °C several times
    Wafer Fabrication Disappearing and broken SU-8 structures Not optimal fabrication procedure; mechanical stress in SU-8 structures Optimize parameter such as baking time, exposure time
    Wafer Fabrication SU-8 layers to low or high or uneven layer thickness Problem during spin coating Check spin-coater parameters and wafer chuck
    Chip Bonding and Assembly  Collapsing PLBRs PDMS bonding parameters not optimal  Adjust power, plasma exposure time. and baking time after bonding
    Chip Bonding and Assembly Dirty structures and particles in the PLBRs Chip was not properly cleaned Apply scotch-tape for surface cleaning 
    Chip Bonding and Assembly Insufficient PDMS-glass bonding Bonding parameters not optimal or insufficient cleaning  Check settings of oxygen plasma
    Microfluidic Experiment Fluid leakage Inlet/outlet hole was not properly punched  Optimize hole punching process
    Microfluidic Experiment Many small PDMS particles during filling Hole was not properly punched  Optimize hole punching process
    Microfluidic Experiment, Biological Aspect No cell growth Solvent residue from cleaning procedure Flush chip more extensively prior cell loading or let solvent evaporate prior bonding
    Microfluidic Experiment, Biological Aspect Changing growth rates Various reasons Check preculture and temperature
    Microfluidic Experiment, Biological Aspect Cell morphology changes during cultivation Nutrient limitations or temperature shift Check incubator and flow
    Microfluidic Experiment, Technical  Aspect Drift in position during time lapse microscopy Temperature fluctuations Check temperature profile prior experiments until no oscillation
    Microfluidic Experiment, Technical  Aspect Loss of cells during cultivation Slightly to high reactor height  Optimize reactor height
    Microfluidic Experiment, Technical  Aspect No trapping Too low reactor height  Optimize reactor height

    Table 1. Troubleshooting. This table summarizes critical aspects, common mistakes, and possible solutions during experimental work.

    Discussion

    We have described the fabrication, experimental setup and related operation procedures of a microfluidic PDMS device containing several (PLBRs) for single-cell analysis of bacteria.

    Microfabrication using soft lithography techniques allows fast adjustments of device dimensions for various sizes and bacteria morphologies. Currently we are optimizing the picoliter bioreactor regarding the cultivation of different microbial organisms and cultivation throughput. In order to increase the trapping efficiency, also the reactor geometry is under optimization. Figure 8 shows four new PLBR devices that are currently validated. In all figures the seeding channel was redesigned with regards to width and shape. In practice, this seems to have an effect on the number of cells that are trapped, but needs further investigations. Significant improvements regarding the trapping efficiency were also achieved by the incorporation of additional overflow channels leading to higher convectional flow through the reactor and more cells being trapped. However, at the same time one increases the risk to wash out cells during cultivation.

    The device is an interesting alternative to macroscale cultivations that have been used for decades to investigate growth and production processes on single-cell level. However, it has some important requirements: For parallel monitoring of several picoliter bioreactors a high resolution and fully motorized microscopic setup with focus drift compensation is mandatory. In addition an incubation system is needed to maintain the desired cultivation temperature constant throughout the measurements.

    We achieve a 95% success rate in device fabrication. Main problems are related to inefficient PDMS-glass bonding, PDMS roof collapse, or fluid leakage (see Table 1 for troubleshooting of most occurring problems). Although the experimental work is done partially under nonsterile conditions, we rarely see contamination during experiments, due the closed fluidic system. PDMS microfluidic devices are optically transparent, therefore, can be used for high resolution in vivo imaging. Although PDMS seems to be perfect for the application, it has a high affinity for hydrophobic molecules, making the use of solvents which are widely used in whole cell biocatalytic processes limited. However, suitable coatings are available to adapt the protocol to these kinds of applications.

    The proposed PLBR is well suited for spatio-temporal analysis of cellular and even subcellular events of various kinds of bacteria. A major advantage of the present approach lies in the ability to quantify microcolony growth directly in contrast to conventional methods. Furthermore, the PLBR allows for culturing under defined and constant conditions. Because the system facilitates the use of small amounts of reagents or materials it carries the advantages of being inexpensive, customizable and amenable to high throughput. In traditional methods average values of the whole population are considered when analyzing microbial cultivation. Furthermore, existing methods need manual sampling which can lead to degradation of samples and thus to errors in the measurement. The PLBR offers new perspectives for bioprocess development and population heterogeneity analysis in microbiology. The PLBR is a promising tool for various applications within bioprocess development and could be applied in various fields of research, e.g. analysis of cell-to-cell heterogeneity, analysis of specific cell clusters within cell-lineages, screening of microbial production strains and real-time investigation of cell phenotypes.

    Disclosures

    The authors have nothing to disclose.

    Acknowledgements

    The authors would like to thank the technician Agnes Müller-Schröer for her valuable contribution. This work was partly performed at Helmholtz Nanoelectronic Facility (HNF) at Forschungszentrum Jülich GmbH. The authors are grateful for the generous help and support.

    Materials

    Name Company Catalog Number Comments
    Silicon wafer 100 mm diameter, P/BOR <100> Si-MAT, Silicon Materials, Germany
    Photoresist SU-8 2000.5 Micro Resist Technology GmbH, Germany
    Photoresist SU-8 2010 Micro Resist Technology GmbH, Germany
    SU-8 Developer mr DEV- 600 Micro Resist Technology GmbH, Germany
    Polydimethylsiloxane (PDMS) Sylgard 184 Silicone Elastomer Kit Dow Corning; Farnell GmbH, Germany
    Dispensing needles Precision Tips  27 G; ID = 0.2 mm, OD = 0.42 mm Nordson EFD Deutschland, Germany
    Glass plates D263 T eco, 30 mm x 25 mm x 0.17 mm Schott AG, Germany
    Hole puncher AKA 5130-B-90 Harris Uni-Core
    Tubing Tygon S-54-HL, ID = 0.25 mm, OD = 0.76 mm Saint Gobain; VWR International GmbH, Germany
    Disposable Syringes – Omnifix Spritzen BRAUN Omnifix 40 Duo, 1 ml B. Braun Melsungen AG, Germany 552-183143
    Syringes, 1 ml sterile glass syringes INNOVATIVE LABOR SYSTEME GMBH (ILS), Germany
    Chemicals
    (NH4)2SO4 Carl Roth GmbH + Co. KG, Germany
    Urea Carl Roth GmbH + Co. KG, Germany
    KH2PO4 Carl Roth GmbH + Co. KG, Germany
    K2HPO4 Carl Roth GmbH + Co. KG, Germany
    MgSO4•7H2O Carl Roth GmbH + Co. KG, Germany
    MOPS Carl Roth GmbH + Co. KG, Germany
    FeSO4•7H2O Carl Roth GmbH + Co. KG, Germany
    MnSO4•H2O Carl Roth GmbH + Co. KG, Germany
    ZnSO4•7H2O Carl Roth GmbH + Co. KG, Germany
    CuSO4 Carl Roth GmbH + Co. KG, Germany
    NiCl2•6H2O Carl Roth GmbH + Co. KG, Germany
    CaCl2 Carl Roth GmbH + Co. KG, Germany
    Biotin Carl Roth GmbH + Co. KG, Germany
    Procatechuic acid Carl Roth GmbH + Co. KG, Germany
    Glucose-Monohydrate Carl Roth GmbH + Co. KG, Germany
    BHI Becton, Dickinson
    Cells
    Corynebacterium glutamicum ATTC 13032 DSMZ; Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany
    Escherichia coli MG 1655 DSMZ; Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany
    Equipment
    Wafer Cleaner SSEC 3300 Solid State Equipment LLC
    Spin Coater SPIN150 -NPP SPS Europe B.V.
    Mask Aligner MA-6 Karl Suess
    Hot Plate HP30A - 2 Torrey Pines Scientific
    Laboratory oven Memmert UN 200 Memmert
    Plasma Cleaner FEMTO Diener Electronics, Germany
    neMESYS syringe pumps Cetoni GmbH, Germany
    Magnetic stirrer CB 162 Stuart VWR 442-0304
    Microscope Nikon Eclipse Ti Nikon Microscopy
    Microscope incubuator Pecon GmbH, Germany
    Centrifuge minispin plus “black line” Eppendorf 9776501
    Photometer BioPhotometer plus  Eppendorf 6132000008
    Shake flask shaker/incubator 3031 GFL - Gesellschaft für Labortechnik mbH, Germany
    Profilometer, Dektak 150 Stylus Profiler Veeco

    References

    1. Muzzey, D., van Oudenaarden, A. Quantitative Time-Lapse Fluorescence Microscopy in Single Cells. Ann. Rev. Cell Dev. Biol. 25, 301-327 (2009).
    2. Wei, P., et al. Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells. Nature. 488, 384-388 (2012).
    3. Park, S., et al. Influence of topology on bacterial social interaction. Proc. Natl. Acad. Sci. U.S.A. 100, 13910-13915 (1073).
    4. Lee, P. J., Helman, N. C., Lim, W. A., Hung, P. J. A microfluidic system for dynamic yeast cell imaging. BioTechniques. 44, 91-95 (2008).
    5. Kobel, S. A., et al. Automated analysis of single stem cells in microfluidic traps. Lab Chip. 12, 2843-2849 (2012).
    6. Carlo, D. D., Wu, L. Y., Lee, L. P. Dynamic single cell culture array. Lab Chip. 6, 1445-1449 (2006).
    7. Locke, J. C. W., Young, J. W., Fontes, M., Jimenez, M. J. H., Elowitz, M. B. Stochastic Pulse Regulation in Bacterial Stress Response. Science. 334, 366-369 (2011).
    8. Boedicker, J. Q., Li, L., Kline, T. R., Ismagilov, R. F. Detecting bacteria and determining their susceptibility to antibiotics by stochastic confinement in nanoliter droplets using plug-based microfluidics. Lab Chip. 8, 1265-1272 (2008).
    9. Fritzsch, F. S. O., et al. Picoliter nDEP traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments. Lab Chip. 13, 397-408 (2013).
    10. Groisman, A., et al. A microfluidic chemostat for experiments with bacterial and yeast cells. Nat. Methods. 2, 685-689 (2005).
    11. Mather, W., Mondragon-Palomino, O., Danino, T., Hasty, J., Tsimring, L. S. Streaming Instability in Growing Cell Populations. Phys. Rev. Lett. 104, (2010).
    12. Wang, P., et al. Robust growth of Escherichia coli. Curr. Biol. 20, 1099-1103 (2010).
    13. Grunberger, A., et al. A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab Chip. 12, 2060-2068 (2012).
    14. Walden, M., Elf, J. Studying transcriptional interactions in single cells at sufficient resolution. Curr. Opin. Biotech. 22, 81-86 (2011).
    15. Keymer, J. E., Galajda, P., Lambert, G., Liao, D., Austin, R. H. Computation of mutual fitness by competing bacteria. Proc. Natl. Acad. Sci. U.S.A. 105, 20269-20273 (2008).
    16. Keymer, J. E., Galajda, P., Muldoon, C., Park, S., Austin, R. H. Bacterial metapopulations in nanofabricated landscapes. Proc. Natl. Acad. Sci. U.S.A. 103, 17290-17295 (2006).
    17. Grunberger, A., et al. Beyond growth rate 0.6: Corynebacterium glutamicum cultivated in highly diluted environments. Biotechnol. Bioeng. 110, 220-228 (2013).
    18. Binder, S., et al. A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level. Genome Biol. 13, (2012).
    19. Mustafi, N., Grunberger, A., Kohlheyer, D., Bott, M., Frunzke, J. The development and application of a single-cell biosensor for the detection of L-methionine and branched-chain amino acids. Metab. Eng. 14, 449-457 (2012).
    20. Huber, R., et al. Robo-Lector - a novel platform for automated high-throughput cultivations in microtiter plates with high information content. Microb. Cell Fact. 8, (2009).
    21. Keilhauer, C., Eggeling, L., Sahm, H. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J. Bacteriol. 175, 5595-5603 (1993).
    22. Frunzke, J., Bramkamp, M., Schweitzer, J. E., Bott, M. Population Heterogeneity in Corynebacterium glutamicum ATCC 13032 caused by prophage CGP3. J. Bacteriol. 190, 5111-5119 (2008).

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