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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Article DOI: 10.3791/50560-v 12:04 min December 6th, 2013
December 6th, 2013



In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.


Microfluidic Picoliter Bioreactor Microbial Single-cell Analysis Fabrication System Setup Operation Microfluidic Cultivation Device CAD Software Polydimethylsiloxane Picoliter Bioreactors Monolayer Growth Microbial Culture Mother Cells Growth Medium Time-lapse Microscopy Corynebacterium Glutamicum Cell-cell Heterogeneity Single Cell Analysis Spatial Resolution Temporal Resolution SU-8 Photolithography Polydimethylsiloxane Chip Molding
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