Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12,366 Views
•
12:04 min
•
December 06, 2013
DOI:
Summary
Automatically generated
In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.
Related Videos
15:41
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Related Videos
14890 Views
11:42
Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
Related Videos
24429 Views
05:10
A Microfluidic Platform to Study Bioclogging in Porous Media
Related Videos
1905 Views
09:51
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
Related Videos
11312 Views
14:25
Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media
Related Videos
10816 Views
10:00
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Related Videos
17569 Views
09:12
Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System
Related Videos
7482 Views
07:05
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
Related Videos
6017 Views
08:19
Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
Related Videos
3518 Views
09:43
Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
Related Videos
1632 Views
Read Article
Cite this Article
Gruenberger, A., Probst, C., Heyer, A., Wiechert, W., Frunzke, J., Kohlheyer, D. Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation. J. Vis. Exp. (82), e50560, doi:10.3791/50560 (2013).
Copy