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Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens
JoVE Journal
Medicine
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JoVE Journal Medicine
Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens
DOI:

05:58 min

February 04, 2014

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Chapters

  • 00:05Title
  • 01:34Reagent Setup
  • 02:00Tissue Processing
  • 04:45Results: Cell Culture Profile
  • 05:37Conclusion

Summary

Automatic Translation

This study describes a detailed method for isolation and characterization of primary ovarian cancer cells from solid clinical specimens. Ovarian cancer clinical specimens are subjected to enzymatic digestion to obtain viable, fibroblast-free epithelial ovarian cancer (EOC) cells highly suitable for downstream applications.

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