05:21Assessing VSMC Calcification Using the Von Kossa Staining Method
06:43Results: MGP Deficiency Results in Vascular Calcification in the Absence of Vascular Inflammation and MGP Protects Cultured VSMCs from Calcification
Vascular calcification is an important predictor of and contributor to human cardiovascular disease. This protocol describes methods for inducing calcification of cultured primary vascular smooth muscle cells and for quantifying calcification and macrophage burden in animal aortas using near-infrared fluorescence imaging.