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JoVE Journal
Genetics

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Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
 

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Article DOI: 10.3791/54752-v 07:40 min October 29th, 2016
October 29th, 2016

Chapters

Summary

The role of assortment (spatial segregation) in evolutionary scenarios can be examined using simple microbial systems in the laboratory that allow controlled adjustment of spatial distribution. By modifying the founder cell density, various assortment levels can be visualized using fluorescently labelled bacterial strains in the colony biofilms of Bacillus subtilis.

Tags

Spatial Segregation Microbial Populations Fluorescent Microscopy Social Microbiology Fitness Assessment Subsegment Image Analysis LB Medium B.subtilis Shaking Incubator Swarming Experiments Sliding Experiments Colony Biofilm Experiments Agar Two Times SG Medium LB Agar
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