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As cells reach confluency, they must be subcultured or passaged. This video will demonstrate a procedure for subculturing both adherent and suspension cells.
Cite this Article
Ricardo, R., Phelan, K. Trypsinizing and Subculturing Mammalian Cells. J. Vis. Exp. (16), e755, doi:10.3791/755 (2008).
The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
The authors have nothing to disclose.
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