18,086 Views
•
13:30 min
•
November 07, 2012
DOI:
Summary
Automatically generated
وقد وضعت رواية الأسلوب التطور الموجه محددة لمجال الهندسة صمود للحرارة وبالتالي التحقق من صحة لالإنزيمات للجراثيم. فقط بعد جولة واحدة من الطفرات العشوائية، وهو الانزيم تطورت للجراثيم، PlyC 29C3، عرض أكبر من النشاط المتبقية مرتين بالمقارنة مع البروتين من النوع البري بعد مرور فترة الحضانة درجة حرارة مرتفعة.
Related Videos
06:03
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Related Videos
14453 Views
09:16
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Related Videos
7317 Views
11:27
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
Related Videos
3998 Views
09:01
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Related Videos
30548 Views
11:25
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Related Videos
6659 Views
06:16
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
Related Videos
20600 Views
10:43
Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
Related Videos
5840 Views
10:50
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Related Videos
10952 Views
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Related Videos
8808 Views
08:11
Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
Related Videos
787 Views
Read Article
Cite this Article
Heselpoth, R. D., Nelson, D. C. A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes. J. Vis. Exp. (69), e4216, doi:10.3791/4216 (2012).
Copy