Articles by Brittany A. Bowman in JoVE
Eficiente de Ácido Nucleico Extração e sequenciamento genético 16S rRNA para Bacteriana Comunidade Caracterização Melis N. Anahtar1, Brittany A. Bowman1, Douglas S. Kwon1 1Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital Nós descrevemos um método eficiente, robusto e de baixo custo para extração de ácidos nucleicos de cotonetes para a caracterização das comunidades bacterianas usando 16S rRNA amplicon gene seqüenciamento. O método permite uma abordagem comum para processamento de múltiplos tipos de amostras e acomoda uma série de processos analíticos jusante.
Other articles by Brittany A. Bowman on PubMed
Cervicovaginal Bacteria Are a Major Modulator of Host Inflammatory Responses in the Female Genital Tract Immunity. May, 2015 | Pubmed ID: 25992865 Colonization by Lactobacillus in the female genital tract is thought to be critical for maintaining genital health. However, little is known about how genital microbiota influence host immune function and modulate disease susceptibility. We studied a cohort of asymptomatic young South African women and found that the majority of participants had genital communities with low Lactobacillus abundance and high ecological diversity. High-diversity communities strongly correlated with genital pro-inflammatory cytokine concentrations in both cross-sectional and longitudinal analyses. Transcriptional profiling suggested that genital antigen-presenting cells sense gram-negative bacterial products in situ via Toll-like receptor 4 signaling, contributing to genital inflammation through activation of the NF-κB signaling pathway and recruitment of lymphocytes by chemokine production. Our study proposes a mechanism by which cervicovaginal microbiota impact genital inflammation and thereby might affect a woman's reproductive health, including her risk of acquiring HIV.
Association Between Injectable Progestin-only Contraceptives and HIV Acquisition and HIV Target Cell Frequency in the Female Genital Tract in South African Women: a Prospective Cohort Study The Lancet. Infectious Diseases. Apr, 2016 | Pubmed ID: 26723758 The use of injectable progestin-only contraceptives has been associated with increased risk of HIV acquisition in observational studies, but the biological mechanisms of this risk remain poorly understood. We aimed to assess the effects of progestins on HIV acquisition risk and the immune environment in the female genital tract.