Other Publications (1)
Articles by Ronil S. Shah in JoVE
A Mouse Model for Laser-induced Choroidal Neovascularization Ronil S. Shah1, Brian T. Soetikno1, Michelle Lajko1, Amani A. Fawzi1 1Department of Ophthalmology, Northwestern University Feinberg School of Medicine Here, we present the mouse laser-induced choroidal neovascularization (CNV) protocol, an experimental model that re-creates the vascular hallmarks of neovascular age-related macular degeneration (AMD). Once mastered, it can reliably and effectively induce CNV as a model system to test various experimental measures.
Other articles by Ronil S. Shah on PubMed
Simultaneous Optical Coherence Tomography Angiography and Fluorescein Angiography in Rodents with Normal Retina and Laser-induced Choroidal Neovascularization Optics Letters. Dec, 2015 | Pubmed ID: 26670511 Fluorescein angiography (FA) is the current clinical imaging standard for vascular related retinal diseases such as macular degeneration and diabetic retinopathy. However, FA is considered invasive and can provide only two-dimensional imaging. In comparison, optical coherence tomography angiography (OCTA) is noninvasive and can generate three-dimensional imaging; investigations of OCTA already demonstrated great promise in retinal vascular imaging. Yet, to further develop and apply OCTA, strengths and weaknesses between OCTA and FA need to be thoroughly compared. To avoid complications in image registration, an ideal comparison requires co-registered and simultaneous imaging by both FA and OCTA. In this Letter, we developed a system with integrated laser-scanning ophthalmoscope FA (SLO-FA) and OCTA, and conducted simultaneous dual-modality retinal vascular imaging in rodents. In imaging healthy rodent eyes, OCTA can resolve retinal capillaries better than SLO-FA does, particularly deep capillaries. In imaging rodent eyes with laser-induced choroidal neovascularization (CNV), OCTA can identify CNV that eludes SLO-FA detection.