1Department of Genetics,Yale School of Medicine, 2Department of Pediatrics,Yale School of Medicine, 3Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, 4Department of Cellular and Molecular Physiology,Yale School of Medicine, 5Yale Stem Cell Center,Yale School of Medicine, 6Yale Cancer Center,Yale School of Medicine
Here, we present a protocol to generate rat intestinal organoids and use them in several downstream applications. Rats are often a preferred preclinical model, and the robust intestinal organoid system fills the need for an in vitro system to accompany in vivo studies.
