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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

June 23rd, 2023
*1, *1,2, 3,4, 1,5,6
1Department of Genetics, Yale School of Medicine, 2Department of Pediatrics, Yale School of Medicine, 3Department of Pediatrics/Gastroenterology and Hepatology, Yale School of Medicine, 4Department of Cellular and Molecular Physiology, Yale School of Medicine, 5Yale Stem Cell Center, Yale School of Medicine, 6Yale Cancer Center, Yale School of Medicine
* These authors contributed equally

Summary
Automatic Translation

Aqui, apresentamos um protocolo para gerar organoides intestinais de ratos e usá-los em várias aplicações a jusante. Os ratos são frequentemente um modelo pré-clínico preferido, e o robusto sistema organoide intestinal preenche a necessidade de um sistema in vitro para acompanhar os estudos in vivo .

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