Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

June 23rd, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen^3,4, Kaelyn Sumigray^1,5,6

¹Department of Genetics, Yale School of Medicine, ²Department of Pediatrics, Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology, Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology, Yale School of Medicine, ⁵Yale Stem Cell Center, Yale School of Medicine, ⁶Yale Cancer Center, Yale School of Medicine

* These authors contributed equally

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כאן, אנו מציגים פרוטוקול ליצירת אורגנואידים במעיים של חולדות ומשתמשים בהם במספר יישומים במורד הזרם. חולדות הן לעתים קרובות מודל פרה-קליני מועדף, ומערכת אורגנואיד המעי החזקה ממלאת את הצורך במערכת חוץ גופית שתלווה מחקרי vivo .

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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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