Advancing Spinal Cord Stimulation - Exploring the Cellular Responses of Motor Neurons Through Patch-Clamp Electrophysiology (Video) | JoVE

Qingyu Yao; Xuesong Luo; Jie Liu; Luming Li

doi:10.3791/65385

Understanding Motor Neuron Responses to Spinal Cord Stimulation

September 8th, 2023

Advancing Spinal Cord Stimulation - Exploring the Cellular Responses of Motor Neurons Through Patch-Clamp Electrophysiology (Video) | JoVE

02:04 min

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Spinal Cord Dissection for the Whole-Cell Patch-Clamp Recording in Motor Neurons

01:44 min

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Preparation of Spinal Cord Slice for the Whole-Cell Patch-Clamp Recording in Motor Neurons (Video) | JoVE

01:15 min

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Patch-Clamp Recording to Study the Electrical Responses of Motor Neurons to Spinal Cord Stimulation (Video) | JoVE

02:34 min

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Qingyu Yao¹, Xuesong Luo¹, Jie Liu^2,3, Luming Li^1,2

¹National Engineering Research Center of Neuromodulation, School of Aerospace Engineering, Tsinghua University, ²IDG/McGovern Institute for Brain Research, Tsinghua University, ³School of Life Sciences, Tsinghua University

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This protocol describes a method using a patch-clamp to study the electrical responses of motor neurons to spinal cord stimulation (SCS) with high spatiotemporal resolution, which can help researchers improve their skills in separating the spinal cord and maintaining cell viability simultaneously.

Article

Understanding Motor Neuron Responses to Spinal Cord Stimulation

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