Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement

Rikako Hirata; Momoko Takagi; Yosuke Toda; Akira Mine

doi:10.3791/66112

Automated Stomatal Response Measurement to Bacterial Invasion in Arabidopsis

February 9th, 2024

Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement

01:12 min

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Induction of <i>Pseudomonas syringae</i> pv. <em>tomato</em> DC3000 Infection in <i>Arabidopsis thaliana</i> for Automated Stomatal Response Measurement

Induction of Pseudomonas syringae pv. tomato DC3000 Infection in Arabidopsis thaliana for Automated Stomatal Response Measurement

01:30 min

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Automated Measurement of Stomatal Response in <i>Arabidopsis thaliana</i> in Response to <i>Pseudomonas syringae</i> pv. <em>tomato</em> DC3000 Infection

Automated Measurement of Stomatal Response in Arabidopsis thaliana in Response to Pseudomonas syringae pv. tomato DC3000 Infection

02:25 min

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Rikako Hirata*¹, Momoko Takagi*², Yosuke Toda^2,3, Akira Mine¹

¹Graduate School of Agriculture, Kyoto University, ²Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, ³Phytometrics Co., Ltd.

* These authors contributed equally

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Here, we present a simple method for direct observation and automated measurement of stomatal responses to bacterial invasion in Arabidopsis thaliana. This method leverages a portable stomatal imaging device, together with an image analysis pipeline designed for leaf images captured by the device.

Article

Automated Stomatal Response Measurement to Bacterial Invasion in Arabidopsis

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