Dharmais Cancer Hospital 1 article published in JoVE Cancer Research The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy Bayu Brahma1, Rizky I. Putri2, Lenny Sari2, Ramadhan Karsono1, Denni J. Purwanto1, Walta Gautama1, Bob Andinata1, Samuel J Haryono1 1Department of Surgical Oncology, Dharmais Cancer Hospital, 2Department of Anatomical Pathology, Dharmais Cancer Hospital In Indonesia, sentinel node biopsy (SNB) is not routinely performed for breast cancer surgery because of the limitation to provide radioisotope tracer and isosulfan or patent blue dye (PBD). To overcome this obstacle, we applied 1% methylene blue dye (MBD) as a single agent to map the sentinel nodes (SNs).