University Children's Hospital 1 article published in JoVE Immunology and Infection Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation Sabrina Fuehner1, Dirk Foell1, Christoph Kessel1 1 This protocol describes a purification method for recombinant tag-free calcium binding protein S100A12 and its ion-induced oligomers for human monocyte stimulation assays.