University of Rome, Tor Vergata View Institution's Website 4 articles published in JoVE Immunology and Infection Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells Alessandra Ventura*1,2, Aaron Vassall*1, Alp Yurter1, Eve Robinson1, Renata Filler1, Douglas Hanlon1, Katrina Meeth1, Harib Ezaldein1, Michael Girardi1, Olga Sobolev1, Marcus W. Bosenberg1,3, Richard L. Edelson1 1Department of Dermatology, Yale University School of Medicine, 2Dermatology Department, University of Rome Tor Vergata, 3Department of Pathology, Yale University School of Medicine The transimmunization (TI) device or plate and related protocols have been developed to replicate the key features of extracorporeal photochemotherapy (ECP), in an experimental setting, allowing for production of physiologically activated, tunable dendritic cells (DCs) for cancer immunotherapy. Medicine Mechanical and Controlled PRP Injections in Patients Affected by Androgenetic Alopecia Pietro Gentile1,2, Simone Garcovich3, Maria Giovanna Scioli4, Alessandra Bielli4, Augusto Orlandi5, Valerio Cervelli6 1Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, 2Chief of Teaching of Plastic and Reconstructive Surgery, Catholic University, 3Dermatology Department, Catholic University, 4Anathomic Pathology Department, University of Rome Tor Vergata, 5Chief of Anathomic Pathology Department, University of Rome Tor Vergata, 6Chief of Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata This paper aims to describe the interfollicular autologous platelet-rich plasma injections (0.20 mL x cm2) in the scalp of 23 patients affected by androgenetic alopecia at a depth of 5 mm using a medical injector gun equipped with a 30 gauge needle, in three sessions. Environment Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants Alice Rotini1,2, Loredana Manfra1,3, Filippo Spanu2, Marco Pisapia1, Anna Maria Cicero1, Luciana Migliore2 1Institute for Environmental Protection and Research (ISPRA), Rome (Italy), 2Department of Biology, Tor Vergata University, Rome (Italy), 3Department of Biology and Evolution of Marine Organisms, Stazione Zoologica, Anton Dohrn, Naples (Italy) This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure. Biology ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection Daniela Romanazzo1, Francesco Ricci1, Silvia Vesco1, Silvia Piermarini1, Giulia Volpe1, Danila Moscone1, Giuseppe Palleschi1 1Department of Sciences and Chemical Technologies, University of Rome, Tor Vergata A protocol to detect trichothecenes (mycotoxins of concern for human health) using a newly developed screening method based on a competitive immunochemical method and a final electrochemical detection is demonstrated.