Taras Shevchenko National University of Kyiv 1 article published in JoVE Chemistry In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer Photopharmacology Kateryna Horbatok*1,2, Tetyana Makhnii*2, Viktoriia Kosach2, Volodymyr Danko1, Andrey Kovalenko1, Stanislav Fatiushchenkov1, Petro Borysko2, Iryna Pishel2, Oleg Babii3,4, Anne S. Ulrich3, Tim Schober4, Sergii Afonin3, Igor V. Komarov1,2,4 1Taras Shevchenko National University of Kyiv, 2Bienta/Enamine, 3Karlsruhe Institute of Technology, 4Lumobiotics This protocol presents a set of experiments adopted for the evaluation of photoswitchable anticancer peptides, that can be used in the preclinical screening of such compounds. This includes cytotoxicity assessment in 2D and 3D cell cultures, the evaluation of ex vivo (model tissue) photoisomerization efficiency, and in vivo efficacy.
