为检验新型抗病毒药物的鉴定对蓝舌病病毒
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Summary
Abstract
Introduction
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| DMEM medium | Gibco | 1134218 | For cell culture |
| FBS | Gibco | 16000044 | For cell culture |
| 0.05% Trypsin-EDTA | Gibco | 1000185 | For cell culture |
| DPBS | Gbico | 1049769 | For cell culture |
| CellTiter-Glo (CTG) kit | Promega | TB288 | For cell viability measurement |
| 70% ethanol | Fisher | S25309B | Diluted from 95% |
| Antiviral huashilcompounds | NIH MLSMR and de novo synthesis | ||
| BTV-10 | ATCC | VR-187 | |
| BSR cell | Developed in house | ||
| Synergy-II multi-mode microplate reader | BioTek | For luminescent signal reading | |
| MicroFlo select dispenser | BioTek | Adding cells, virus, and reagents | |
| 384-well flat-bottom microplate | CORNING | 28908031 | For cell culture |
| Gen. 5 software | BioTek | For analysis of reading outputs from Synergy-II multi-mode microplate reader | |
| GraphPad Prism 5 | GraphPad | Version 5 | For biostatic analysis and plot |
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Cite This Article
Gu, L., Schneller, S. W., Li, Q. Assays for the Identification of Novel Antivirals against Bluetongue Virus. J. Vis. Exp. (80), e50820, doi:10.3791/50820 (2013).