Protocol
Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics
Pavel Mokrejš1, Matouš Huťťa1, Jana Pavlačková2, Pavlína Egner2
1Department of Polymer Engineering, Faculty of Technology, Tomas Bata University in Zlín, 2Department of Fat, Tenside and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlín
The goal of the protocol is to prepare keratin hydrolysate from chicken feathers by alkaline-enzymatic hydrolysis and to test whether adding keratin hydrolysate into a cosmetics ointment base improves skin barrier function (heightening hydration and diminishing transepidermal water loss). Tests are conducted on men and woman volunteers.
Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Kaiwen Sun*, Yuyu Zheng*, Ziqiang Zhu
This manuscript describes an easy and rapid experimental procedure for determining protein-protein interactions based on the measurement of luciferase activity.
Wind Tunnel Experiments to Study Chaparral Crown Fires
Jeanette Cobian-Iñiguez1, AmirHessam Aminfar1, Joey Chong2, Gloria Burke2, Albertina Zuniga1, David R. Weise2, Marko Princevac1
1Department of Mechanical Engineering, University of California, Riverside, 2Pacific Southwest Research Station, USDA Forest Service
This protocol describes wind tunnel experiments designed to study the transition of a fire from the ground to the canopy of chaparral shrubs.
Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System
Steven E. Naranjo1, Peter C. Ellsworth2
1USDA-ARS, Arid-Land Agricultural Research Center, 2Department of Entomology, Maricopa Agricultural Center, University of Arizona
Life tables allow quantification of the sources and rates of mortality in insect populations and contribute to understanding, predicting and manipulating population dynamics in agroecosystems. Methods for conducting and analyzing cohort-based life tables in the field for an insect with sessile immature life stages are presented.
Disclosures
No conflicts of interest declared.