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Görselleştirme Antijen spesifik CD4 + T hücreleri MHC Sınıf II Tetramers kullanarak

Article DOI: 10.3791/1167-v • 15:43 min • March 6th, 2009
March 6th, 2009 •
1, 2, 3, 2
1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute

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Bu prosedür, arıtma gösteriyor ve in vitro antijen spesifik CD4 + T hücreleri, tüm periferik kan ve MHC sınıf II tetramers kullanarak görselleştirme genişleme. Tetramers tek bir antijen özgüllüğü ve tanımlanan MHC sınıf II kısıtlama ile T hücrelerinin doğrudan görselleştirme izin verir.

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İmmünoloji Sayı 25 CD4 + T hücre in vitro genişleme MHC sınıf II tetramers periferik kan mononükleer hücreler flow sitometri
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