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DNA Transfectie van zoogdieren skeletspieren met behulp van In Vivo Elektroporatie

Article DOI: 10.3791/1520-v • 15:56 min • October 19th, 2009
October 19th, 2009 •
1, 1, 1, 1
1Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles

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We beschrijven gedetailleerde procedures voor de efficiënte transfectie van plasmide DNA in de vezels van mond spieren van levende muizen met behulp van elektroporatie en de daaropvolgende visualisatie van eiwitexpressie met behulp van fluorescentie microscopie.

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Cellular Biology elektroporatie skeletspieren plasmiden eiwit expressie muis twee-foton microscopie fluorescentie transgene
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