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Chapters
- 00:00Title
- 00:48Introduction
- 01:12Excision and Long-Term Culture of Animal Cap Explants
- 03:39High-Resolution Live-Imaging Confocal Microscopy
- 04:30Image Analysis
- 08:27Conclusion
Xenopus embryonic epithelia are an ideal model system to study cell behaviors such as polarity development and shape change during epithelial morphogenesis. Traditional histology of fixed samples is increasingly being complemented by live-cell confocal imaging. Here we demonstrate methods to isolate frog tissues and visualize live epithelial cells and their cytoskeleton using live-cell confocal microscopy.
Tags
Live-cell ImagingQuantitative AnalysisEmbryonic Epithelial CellsXenopus LaevisMorphogenesisCell Surface AreaCell HeightMitosisMigrationMorphogenetic MovementsChemical FixationHistological MethodsCell MorphologyProtein LocalizationHigh Resolution Live ImagingMulticellular Epithelial SheetsConfocal Imaging
