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Journal / Bioengineering / Organotypische Collagen I Assay: A Temperguss…
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Bioengineering

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JoVE Journal Bioengineering
Organotypische Collagen I Assay
Biofunctionalization of Magnetic Nanomaterials
Journal (Bioengineering)
Biofunctionalization of Magnetic Nanomaterials
Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications
Journal (Bioengineering)
Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications
 
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Organotypische Collagen I Assay: A Temperguss Platform to Cell Verhalten in 3-Dimensional Kontext beurteilen

Article DOI: 10.3791/3089-v • 10:46 min • October 13th, 2011
October 13th, 2011 •
1, 1, 1, 1, 2, 2, 1
1The Beatson Institute for Cancer Research, University of Glasgow, 2Section of Dermatology, School of Medicine, University of Glasgow

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Es wird ein Verfahren zur Herstellung einer 3-dimensionalen Matrix, bestehend aus Kollagen Typ I und primären humanen Fibroblasten beschrieben. Diese organotypischen Gel dient als nützliches Substrat invasive Zellmigration beurteilen, da es grundlegende Funktionen von Gewebestroma nachahmt und ist offen für viele Formen der Mikroskopie.

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Bioengineering Organotypische Kultur Zellmigration Invasion 3-dimensionale Matrix Kollagen I Second Harmonic Generation Host-Tumor-Interaktion Mikroumgebung
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