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Metabolsk Pathway Bekreftelse og Discovery Through 13 C-merking av Proteinogenic Amino Acids
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Metabolsk Pathway Bekreftelse og Discovery Through 13 C-merking av Proteinogenic Amino Acids

Article DOI: 10.3791/3583-v • 07:27 min • January 26th, 2012
January 26th, 2012 •
1, 2, 1, 1, 3, 1
1Department of Energy, Environmental and Chemical Engineering, Washington University, 2Department of Biology, Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology, Washington University

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13 C-isotopen merking er en nyttig teknikk for å bestemme cellen sentrale metabolisme for ulike typer av mikroorganismer. Etter at cellene har vært dyrket med en bestemt merket substrat, kan GC-MS måling avdekker funksjonelle metabolismeveier basert på unike merking mønstre i proteinogenic aminosyrer.

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Molecular Biology GC-MS roman vei metabolisme merking phototrophic mikroorganisme
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