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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput Detection Method for influensavirus
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High-throughput Detection Method for influensavirus

Article DOI: 10.3791/3623-v • 10:05 min • February 4th, 2012
February 4th, 2012 •
1, 1, 2, 3, 4, 4, 1,5, 1,6
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

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Denne metoden beskriver bruk av Infrarød fargestoffbaserte imaging system for påvisning av H1N1 i bronchioalveolar lavage (BAL) væske av infiserte mus på en høy sensitivitet. Denne metodikken kan utføres i en 96 - eller 384-brønnen plate, krever <10 mL volum av testmateriale og har potensial for samtidige screening av flere patogener.

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Immunologi Influensa virus Virus titer Epitelceller
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