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代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体

JoVE 杂志
生物学

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JoVE 杂志生物学

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

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代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体

DOI:

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12:03 min

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October 31, 2012

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Andreia Gianotti Sommer, Sarah S. Rozelle, Spencer Sullivan, Jason A. Mills, Seon-Mi Park, Brenden W. Smith, Amulya M. Iyer, Deborah L. French, Darrell N. Kotton, Paul Gadue, George J. Murphy, Gustavo Mostoslavsky

¹Center for Regenerative Medicine (CReM),Boston University School of Medicine, ²Department of Hematology,Children’s Hospital of Philadelphia, ³Center for Cellular and Molecular Therapeutics,Children’s Hospital of Philadelphia

Chapters

00:05Title
01:50Isolation and Expansion of PBMCs
04:34Transduction of PBMCs with STEMCCA Lentivirus
06:11Plating Transduced Cells onto MEFs
08:41Picking and Expansion of iPSC Clones
09:26Results: Human Induced Pluripotent Stem Cells
11:26Conclusion

Summary

自动翻译

这里，我们表明一个简单而有效的协议，用于从外周血3-4毫升，使用一个单一的慢病毒重新编程矢量代人类iPSCs。现成的血细胞重新编程的承诺，加快利用IPSC的技术，使其接触到更广泛的研究团体。

Tags

Human Induced Pluripotent Stem Cells Peripheral Blood STEMCCA Lentiviral Vector Transcription Factors Oct4 Klf4 Sox2 CMyc Pluripotent State Embryonic Stem Cells Immune Rejection Disease Modeling Studies Pharmacological Compounds Screening Regenerative Therapies Transgene-free IPSCs Lung Diseases Skin Fibroblasts Reprogramming Peripheral Blood Cells

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