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Rapid Genetic Analysis of epitelial-mesenchymale signaleringsprotokoll Under Hair Regeneration
JoVE 杂志
生物学
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JoVE 杂志 生物学
Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Rapid Genetic Analysis of epitelial-mesenchymale signaleringsprotokoll Under Hair Regeneration

DOI:
10.3791/4344-v

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10:09 min

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February 28, 2013

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, , ,
1Program in Epithelial Biology,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 01:31Isolating and Preparing Mouse Skins
  • 03:17Isolating Primary Dermal or Epidermal Cells from Skins
  • 05:07Grafting Primary Dermal and Epidermal Cells
  • 07:57Histological Analysis of Hair Follicle Growth
  • 09:32Conclusion

Summary

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Vev-spesifikk analyse av en hårsekken regenerering modellen bruker lentivirus å megle gevinst-eller tap av funksjon.

Tags

Genetic AnalysisEpithelial-mesenchymal SignalingHair RegenerationHair Follicle MorphogenesisOrgan DevelopmentLentivirusOverexpressionShRNA-mediated KnockdownGain-of-function AnalysisLoss-of-function AnalysisPrimary Mouse KeratinocytesDermal CellsDermal Papilla CellsHair FolliclesNude MiceGenetic Models

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