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Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
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Neuroscience
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JoVE Journal Neuroscience
Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

DOI:
10.3791/50253-v

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08:13 min

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March 11, 2013

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, , ,
1Department of Physiology,David Geffen School of Medicine at UCLA, 2Natural Science Division,Pepperdine University

Chapters

  • 00:05Title
  • 01:41Pre-experimental Preparation
  • 03:24Microdissection of Xenopus embryos
  • 04:51Preparation of Nerve-muscle Co-cultures
  • 05:52Patch Clamping Nerve-muscle Synapses
  • 07:02Results: Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.

Tags

Keywords: XenopusNerve-muscle Co-cultureElectrophysiologyPresynapticPostsynapticNeurotransmitter ReleaseIon ChannelsSynaptic InteractionsSynaptic DevelopmentSynaptic Plasticity

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