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Genetisk kodede Molecular Probes at studere G-protein-koblede receptorer

Article DOI: 10.3791/50588-v • 16:16 min • September 13th, 2013
September 13th, 2013 •
1, 1, 1, 1, 1
1Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University

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Vi genetisk kode for den unaturlige aminosyre, p-azido-L-phenylalanin på forskellige målrettede positioner i GPCR'er og vise alsidigheden af ​​azidogruppen i forskellige applikationer. Heriblandt en målrettet fototværbinding teknologi til at identificere rester i den ligandbindende lomme af en GPCR, og stedspecifik bioorthogonal modificering af GPCR'er med et peptid-epitop-mærke eller fluorescerende probe.

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Genetics receptorer G-protein-koblede Protein Engineering signaltransduktion Biochemistry unaturlig aminosyre steddirigeret mutagenese G-protein-koblet receptor målrettet fototværbinding bioorthogonal mærkning målrettet epitop tagging
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