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Journal / Immunology and Infection / Rekombinante Expression von Funktions…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Rekombinante Expression von Funktions Hämagglutinin und Neuraminidase-Proteine ​​aus dem Roman H7N9 Influenza-Virus mit dem Baculovirus-Expressionssystem
Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
Journal (Immunology and Infection)
Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
Journal (Immunology and Infection)
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
 
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Rekombinante Expression von Funktions Hämagglutinin und Neuraminidase-Proteine ​​aus dem Roman H7N9 Influenza-Virus mit dem Baculovirus-Expressionssystem

Article DOI: 10.3791/51112-v • 12:18 min • November 6th, 2013
November 6th, 2013 •
1,2, 1,3, 1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai

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Hier beschreiben wir einen Weg, korrekt gefaltete und funktionelle Influenza-Virus-Oberflächenantigene von den neuen chinesischen H7N9-Virus in Insektenzellen abgeleitet auszudrücken. Die Technik lässt sich an beliebigen Ektodomänen viraler oder zellulärer Oberflächenproteine ​​exprimieren.

Tags

Infektion Influenza A-Virus, Grippe Mensch Influenza bei Vögeln Influenza-Impfstoffe Hämagglutinin Neuraminidase H7N9 Baculovirus Insektenzellen Expression rekombinanter Proteine
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