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Immunology and Infection

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Metodologia per la generazione efficiente di proteine del virus vaccinia taggate fluorescentmente

Article DOI: 10.3791/51151-v • 09:27 min • January 17th, 2014
January 17th, 2014 •
*1, *1, 1, 2, 3, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Medicine, Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science, University of Melbourne
* These authors contributed equally

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Qui viene descritto un protocollo rapido e modulare per la generazione di virus vaccinia ricombinanti che esprimono simultaneamente proteine contrassegnate fluorescentmente utilizzando il metodo della selezione dominante transitoria.

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Immunologia e infezione Numero 83 virus vaccinia proteina fluorescente virus ricombinante selezione dominante transitoria imaging trasporto subcellulare
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