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Kvantificering af Breast Cancer Cell invasiv Ved hjælp af en tre-dimensionel (3D) Model

Article DOI: 10.3791/51341-v • 08:08 min • June 11th, 2014
June 11th, 2014 •
*1, *1, 1,2,3
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Oncology, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Lawson Health Research Institute
* These authors contributed equally

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Denne artikel indeholder detaljerede metoder til anvendelse af tre-dimensionelle (3D) assays at kvantificere brystkræft celle invasion. Konkret har vi diskutere de procedurer, der kræves for at oprette sådanne assays, kvantificering og dataanalyse, samt metoder til at undersøge tabet af membranintegritet der opstår, når celler invadere.

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Medicine Brystkræft celle invasion ekstracellulær matrix (ECM) tre-dimensionelle (3D) kulturer immuncytokemi Matrigel basalmembranmatrix
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