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Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
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医学
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JoVE 杂志 医学
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model

15,658 Views

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08:08 min

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June 11, 2014

DOI:

10.3791/51341-v

DOI:
10.3791/51341-v

•

08:08 min
June 11, 2014

•

5 Views
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1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario, 2Department of Oncology, Schulich School of Medicine and Dentistry,University of Western Ontario, 3Lawson Health Research Institute

Summary

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This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade.

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Cite this Article

Cvetković, D., Goertzen, C. G., Bhattacharya, M. Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model. J. Vis. Exp. (88), e51341, doi:10.3791/51341 (2014).

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