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Gecombineerde DNA-RNA-TL
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Gecombineerde DNA-RNA-TL

Article DOI: 10.3791/51628-v • 15:54 min • June 14th, 2014
June 14th, 2014 •
1, 1
1Department of Reproduction and Development, Erasmus MC - University Medical Center

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Fluorescente in situ hybridisatie (FISH) maakt de detectie van nucleïnezuren in hun eigen omgeving binnen cellen. We beschrijven hier een protocol voor gecombineerde, gelijktijdige detectie van RNA en DNA door middel van FISH, die kan worden gebruikt om X-chromosoom inactivatie in muis embryonale stamcellen.

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Biochemie TL gecombineerde DNA-RNA FISH ES-cel cytogenetica single cell analyse X-chromosoom inactivatie (XCI), Bacteriële kunstmatig chromosoom (BAC) DNA-probe,
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