需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Chapters
- 00:05Title
- 01:47Screening for Supression of Proteotoxicity
- 03:045–FOA Counter–selection and Spotting to Eliminate False Positives
- 05:41Sequencing Hsp104 Variants by Colony PCR
- 06:45Results: Representative Hsp104 Toxicity Suppresstion Screening
- 07:53Conclusion
Yeast proteinopathy models are valuable tools to assess the toxicity and aggregation of proteins implicated in disease. Here, we present methods for screening Hsp104 variant libraries for toxicity suppressors. This protocol could be adapted to screen any protein library for toxicity suppressors of any protein that is toxic in yeast.
