An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Starlyn L. M. Okada; Nicole S. Stivers; Peter K. Stys; David P. Stirling

doi:10.3791/52173

Journal

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神经科学

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一个离体激光诱导脊髓损伤模型，以评估轴索变性的机制在实时

An <em>Ex Vivo</em> Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

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神经科学

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JoVE 杂志神经科学

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

一个离体激光诱导脊髓损伤模型，以评估轴索变性的机制在实时

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10,862 Views

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11:18 min

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November 25, 2014

DOI:

10.3791/52173-v

DOI:

10.3791/52173-v

•

11:18 min

November 25, 2014

•

10839 Views

Starlyn L. M. Okada¹, Nicole S. Stivers¹, Peter K. Stys², David P. Stirling¹

¹KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville ²Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

Summary

We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.