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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Article DOI: 10.3791/52332-v • 11:05 min • January 21st, 2015
January 21st, 2015 •
*1, *1, 2, 3,4, 5, 2, 2, 2, 2
1Department of Bioengineering, University of Illinois at Chicago, 2Department of Pathology, University of Illinois at Chicago, 3Department of Biological Sciences, University of Illinois at Chicago, 4Department of Chemistry, University of Illinois at Chicago, 5Department of Nephrology, University of Illinois at Chicago
* These authors contributed equally

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Fourier Transform Infrared (FT-IR) spectroscopic imaging is a fast and label-free approach to obtain biochemical data sets of cells and tissues. Here, we demonstrate how to obtain high-definition FT-IR images of tissue sections towards improving disease diagnosis.

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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging Pathology Improvement Detailed Images Biochemical Information Mid-infrared Absorption Chemical Bonds Biomolecules Composition FT-IR Image IR Spectrum Biochemical Status Cell-type Classification Disease-type Classification FT-IR System High-definition Imaging Capabilities Visualization Of FT-IR Images Applications In Pathology Liver Kidney Label-free Analysis
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