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Immunology and Infection

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JoVE Journal Immunology and Infection
Vereinfachte Menschen Neutrophil Extracellular Traps (NETs) Isolierung und Handhabung
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Vereinfachte Menschen Neutrophil Extracellular Traps (NETs) Isolierung und Handhabung

Article DOI: 10.3791/52687-v • 09:43 min • April 16th, 2015
April 16th, 2015 •
*1, *1, 1, 1, 1
1LD MacLean Surgical Research Laboratories, Department of Surgery, McGill University
* These authors contributed equally

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Im folgenden Protokoll beschreiben wir eine sehr einfache Möglichkeit, Neutrophil Extracellular Traps (NETs) aus humanem Vollblut unter Verwendung leicht verfügbarer Reagenzien zu isolieren. Dann zeigt, wie die isolierten Netze können in einem in vitro-Adhäsionstest mit Krebszellen verwendet werden.

Tags

Immunologie Neutrophile Neutrophilen Extracellular Traps Entzündungen Bakterien Infektionen Krebs Metastasen die Haftung
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