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1Department of Cardiovascular Surgery, German Heart Center Munich,Technische Universität München, 2Institute for Medical Microbiology, Immunology, and Hygiene,Technische Universität München, 3Clinical Cooperation Groups: “Antigen-specific Immunotherapy” and “Immune Monitoring”, Helmholtz Center Munich (Neuhererg),Technische Universität München, 4DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance
Chapters
- 00:05Title
- 01:41Cell Culture Treatment with Nanoparticles
- 03:42Identification of Cy3-positive Cells via Flow Cytometry
- 05:53Results: Detection of Pluripotency Marker Gene Expression in Live iPSCs and Embryonic Stem Cells
- 07:36Conclusion
This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.
