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Métodos para descubrir uso promotor alternativo y la regulación transcripcional de murino BCRP1

Article DOI: 10.3791/53827-v • 11:02 min • May 27th, 2016
May 27th, 2016 •
1,2, 1,3, 4, 5,6, 7, 1,3,8,9, 1,3,8,9,10,11
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Pharmaceutical Sciences, University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, 6Magee Women's Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, 8Medicine, University of Maryland School of Medicine, 9Pathology, University of Maryland School of Medicine, 10Pharmacology, University of Maryland School of Medicine, 11Experimental Therapeutics, University of Maryland School of Medicine

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Con el murino transportador ABC BCRP1 (Abcg2) como un ejemplo, en-silico protocolos se presentan para detectar el uso de promotor alternativo en los genes expresados ​​en tejidos de ratón, y para evaluar la funcionalidad de los promotores alternativos identificados mediante los ensayos de reportero.

Tags

Medicina No. 111 Promotor BCRP1 el uso promotor alternativo E1U testículo murino el factor-1 androgénica
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