Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

00:05Title
00:58Isolation of Nuclei
03:54Preparation of HeLa-S3 Nuclear Salt-extractable and Chromatin-bound Fractions
07:05Protein Complexes Purification
11:22Results: MyoD Interactors Identified by Tandem Affinity Purification Coupled to Mass Spectrometry
13:37Conclusion

Summary

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MyoD is a myogenic transcription factor with a strong capacity to induce myogenic transdifferentiation of many fully differentiated non-muscle cell lines. The epigenetic mechanisms involved in this transdifferentiation are largely unknown. Here we describe a double-affinity purification method followed by mass spectrometry to exhaustively characterize MyoD partners.

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