Journal
/
生物学
/
Nephrotoxin Mikroinjektion i zebrafisk til model akut nyreskade
JoVE 杂志
生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

Nephrotoxin Mikroinjektion i zebrafisk til model akut nyreskade

DOI:
10.3791/54241-v

•

07:58 min

•

July 17, 2016

•

,
1Center for Zebrafish Research, Department of Biological Sciences,University of Notre Dame, 2Center for Stem Cells and Regenerative Medicine, Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 00:32Preparation of Tools
  • 02:09Embryo Preparation
  • 03:59Microinjection of Nephrotoxin Solution
  • 06:32Results: Experimental Timeline and Post Injection Edema Assay
  • 07:34Conclusion

Summary

自动翻译

自动翻译

Nyreskader afholdt fra nefrotoksiner, som omfatter lægemidler, der spænder fra antibiotika til kemoterapeutika, kan resultere i komplekse sygdomme, hvis patogenese forbliver ufuldstændigt forstået. Denne protokol viser, hvordan zebrafisk kan anvendes til sygdom modellering af disse forhold, som kan anvendes til identifikation af renoprotective foranstaltninger.

Tags

ZebrafishAcute Kidney InjuryNephrotoxinGentamicinCisplatinEmbryoMicroinjectionAgaroseE3MatingSpawningEmbryo CollectionIncubationSomite Stage

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Transplantation of Whole Kidney Marrow in Adult Zebrafish

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Microinjection of Zebrafish Embryos to Analyze Gene Function

Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Dissection of the Adult Zebrafish Kidney

Laser-Påført skade af zebrafisk Embryonale skeletmuskulatur

Analyse af Nephron opbygning og funktion i Voksen Zebrafisk Kidney

Evaluering af Zebrafisk nyrefunktion Ved hjælp af en fluorescerende Clearance Assay

Read Article