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Un Mimic del microambiente tumorale: Un metodo semplice per la generazione di popolazioni di cellule arricchito e Investigating intercellulare Comunicazione
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
DOI:

09:52 min

September 20, 2016

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Chapters

  • 00:05Title
  • 00:52Insert Preparation
  • 03:40Cell Collection and In Situ Immunofluorescence Characterization
  • 06:22Results: Representative Tumor Microenvironment Generation
  • 07:47Conclusion

Summary

自动翻译

We adapted a permeable microporous membrane insert to mimic the tumor microenvironment (TME). The model consists of a mixed cell culture, allows simplified generation of highly enriched individual cell populations without using fluorescent tagging or cell sorting, and permits studying intercellular communication within the TME under normal or stress conditions.

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