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Journal / Biochemistry / 荧光各向异性作为一种工具来研究蛋白质相互作用…
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JoVE Journal Biochemistry
荧光各向异性作为一种工具来研究蛋白质相互作用
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Journal (Biochemistry)
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Journal (Biochemistry)
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
 
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荧光各向异性作为一种工具来研究蛋白质相互作用

Article DOI: 10.3791/54640-v • 10:44 min • October 21st, 2016
October 21st, 2016 •
1, 2, 1
1Departamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, 2Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México

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蛋白质相互作用是在一个细胞的功能的心脏。量热和光谱技术通常用于表征它们。这里我们介绍荧光各向异性作为一种工具来研究在Shwachman-Diamond综合征（SBDS）突变蛋白质和延伸因子样1 GTP酶（EFL1）之间的相互作用。

Tags

生物化学，第116，荧光各向异性，荧光，延伸因子样1，Shwachman-Diamond综合征蛋白质，蛋白质相互作用，荧光基团
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