解决由蓝变性PAGE和蛋白质相关剖析亲和纯化的蛋白复合物
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09:35 min
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April 01, 2017
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Summary
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这里，我们提出使用无标记的定量质谱法对蛋白复合物的亲和纯化和由蓝色天然PAGE它们的分离协议，其次是蛋白相关性分析。这种方法是非常有用的解决相互作用组分成不同的蛋白复合物。
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Cite this Article
Pardo, M., Bode, D., Yu, L., Choudhary, J. S. Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling. J. Vis. Exp. (122), e55498, doi:10.3791/55498 (2017).
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