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נייד דור גזע מושר מתאי דם באמצעות וירוס Sendai ו צנטריפוגה
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
DOI:

09:57 min

December 21, 2016

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Chapters

  • 00:05Title
  • 01:09Isolation of Monocytic Cells from Blood
  • 02:40Transduction by Sendai Virus
  • 05:46Reprogrammed Cell Maintenance
  • 07:20Results: Bright-field Image and Pluripotency of iPSCs Generated from PBMCs
  • 08:39Conclusion

Summary

自动翻译

We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.

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