Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Yeri Alice Rim; Yoojun Nam; Ji Hyeon Ju

doi:10.3791/54650

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Inducida por la generación de células madre pluripotentes a partir de células de la sangre usando el virus Sendai y centrifugación

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

JoVE Revista
Biología del desarrollo

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JoVE Revista Biología del desarrollo

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Inducida por la generación de células madre pluripotentes a partir de células de la sangre usando el virus Sendai y centrifugación

Please note that all translations are automatically generated. Click here for the English version.

14,177 Views

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09:57 min

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December 21, 2016

DOI:

10.3791/54650-v

DOI:

10.3791/54650-v

•

09:57 min

December 21, 2016

•

12 Views

Yeri Alice Rim¹, Yoojun Nam¹, Ji Hyeon Ju²

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine,The Catholic University of Korea

Transcripción

Summary

We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.