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Journal / Immunology and Infection / Generating
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Immunology and Infection

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Neutrophil Isolation and Activation
Journal (Immunology and Infection)
Neutrophil Isolation and Activation
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
Journal (Immunology and Infection)
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
 
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Generating

Article DOI: 10.3791/54697-v • 08:48 min • October 25th, 2016
October 25th, 2016 •
1,2, *2, *2, 2, 1,2, 1,2, 2, 2, 1,2, 1,2
1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network
* These authors contributed equally

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Hierin beschrijven we een nieuwe methode om antigeenspecifieke T-celreceptoren (TCR's) door het koppelen van de TCRα of TcR van een bestaande TCR, bezit de antigeen-specificiteit van interesse, met aanvullende hemichain van de perifere T-cel receptor repertoire genereren. De novo geproduceerde TCRs behouden antigeen-specificiteit met verschillende affiniteit.

Tags

Immunologie T cel-antigeen-presenterende cel T-celreceptor hemichain retrovirale transductie antigeen-specificiteit humane cloning
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